This peer-reviewed study, published in the International Journal of Molecular Sciences, explores the use of a probiotic-based topical spray (LUCAA+ Dermo Care) to accelerate wound healing in canine skin cells – and the results are promising.

Using a scratch test on cultured dog keratinocyte cells (CPEK), scientists applied various concentrations of both formulated and non-formulated probiotic cocktails. The probiotic-treated cells closed their “wounds” up to 20% faster than untreated ones – with the best results seen in the fully formulated spray, suggesting a synergistic effect between the probiotics and the spray base.

Importantly, the probiotics were well tolerated at low and moderate concentrations, and they activated a beneficial immune response by increasing cytokines like IL-8 and IL-6 – both key to healthy wound healing. These early signals prompt cell repair, promote immune protection, and help balance the skin’s microbiome.

This was the first time a scratch wound assay was used on canine keratinocytes to assess probiotics for veterinary skin care. The study lays a scientific foundation for developing non-medicated probiotic sprays that support natural healing in pets without disrupting the skin barrier or contributing to antimicrobial resistance.

Further testing in 3D models and live animals is needed, but the research strongly supports the potential of probiotics as a safe, effective wound care solution for dogs.

Read the full article to learn how this microbiome-friendly solution offers a promising approach for wound management while reducing antimicrobial resistance risks.

Citation
Barthe, M.; Gillot, L.; Perdigon, L.; Jacobs, A.; Schoonbroodt, G.; Mauhin, P.; Bouhajja, E.; Osman-Ponchet, H. Topical Probiotic
Formulation Promotes Rapid Healing in Dog Keratinocyte Cells: A Promising
Approach for Wound Management. Int. J. Mol. Sci. 2023, 24,
12360. https://doi.org/10.3390/ijms241512360.